DOT and NON DOT Drug Testing



DOT Drug Tests: These are federally regulated DOT drug tests required for CDL holders and other safety-sensitive positions under agencies like FMCSA, FAA, FTA, PHMSA, USCG, and FRA. DLT is commonly used as a lab or billing code for DOT 5-panel urine tests compliant with 49 CFR Part 40.



Non-DOT Drug Tests: These tests are commonly used for employers who want to conduct pre-employment or random drug screening outside of federal regulations. While still using secure chain of custody procedures,  Non-DOT tests are not regulated by FMCSA or DOT, and testing requirements are set by the employer or organization.